POLINIZACIÓN ESTIGMÁTICA IN VITRO DE FRESA (FRAGARIA VESCA) A PARTIR DE LAS VARIEDADES MONTEREY, ALBEÓN Y DIAMANTE CON MIRAS A LA OBTENCIÓN DE UN NUEVO GENOTIPO

Authors

  • Karla Rivera Universidad Tecnologica Equinoccial
  • Alejandra Rivera Universidad Tecnologica Equinoccial
  • Carlos Peña Universidad de las Fuerzas Armadas (ESPE)

DOI:

https://doi.org/10.29019/tsafiqui.v0i3.221

Keywords:

in vitro, pollen, pollination, stigma, pollen tube

Abstract

The strawberry sowing is a really important issue in Ecuador, because these plantations are increasing around the country. It has seen the necessity to have an own variety to improve internal production. The goal of this investigation is to get a new genotipe from varieties that already exitst in camp. First of all three varieties of elit plants were isolated in greenhouse conditions and they were induced to following blooms with Giberelic acid. Regularly open Flowers were emasculated, later they were introduced to in vitro conditions. How ever, before they were desinfected with a sodium hipochlorite solution 0.5% and fungicide 1.5%. The pistils were evaluated in 6,8,10 and 15 minutes, later petals, sepals and anthers were eliminated; they were introduced in M&S in vitro médiums. The oxidation and the presence of exudate were valorated at the base of the stigma. Prove of viability were made in the male gamets (Pollen) with pollen tube germination. It was made from anthers with out dehiscence, the inmature pollen was able to germnated in a BK medium with 500 mg/L of boron and sucrose 10%, gettin polinic longers and helthly tubes at 12 hours of incubation, This investigation is the base to look for a new genotip from in vitro procces with two varities.

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How to Cite

Rivera, K., Rivera, A., & Peña, C. (2012). POLINIZACIÓN ESTIGMÁTICA IN VITRO DE FRESA (FRAGARIA VESCA) A PARTIR DE LAS VARIEDADES MONTEREY, ALBEÓN Y DIAMANTE CON MIRAS A LA OBTENCIÓN DE UN NUEVO GENOTIPO. Tsafiqui, 3(3), 53–60. https://doi.org/10.29019/tsafiqui.v0i3.221